The Complete Beginner’s Guide to Microneedling with a 0.25mm 540 Needle Derma Roller

Contact Us
a woman uses derma roller on her face

Microneedling is rapidly growing in popularity for its ability to improve the texture, tone, and appearance of skin. As leaders in medical aesthetic supplies, we at Face Med Store want to provide you with a complete guide on using microneedle rollers for skin rejuvenation at home. 

This time, we’ll focus on the surgical stainless steel 540 needle derma roller with a 0.25mm needle length. Keep reading as we share our expert advice on how this device can help you achieve radiant, youthful-looking skin.

What is the purpose of using a surgical stainless steel derma roller with 540 needles at 0.25mm length?

The purpose of using a surgical stainless steel derma roller with 540 needles at 0.25mm length is primarily to stimulate collagen production and gently exfoliate the top layer of skin. 

To prove this, a study by Lima et al. (2017) found that microneedling with derma rollers can improve the appearance of scars, including acne scars, by promoting collagen induction and skin remodeling.

The tiny 0.25mm microneedles create micro-punctures in the skin that signal your body to generate new collagen and elastin. This boosts skin thickness and firmness for a smoother, more youthful appearance. The needles also slough off dead cells on the surface for enhanced radiance and product absorption.

As skincare experts, we recommend microneedle rollers with 0.25mm needles for those new to the treatment who want subtle rejuvenation. The short needle length is less invasive than longer sizes and ideal for sensitive skin types. The 540 needle design ensures thorough coverage and even distribution when rolled across the face

Surgical stainless steel offers durability and strength for reliable results without irritation. With regular use, you can enjoy refined texture, minimize pores, reduced wrinkles and acne scars, fading of stretch marks, and an all-around glow.

How do I choose the right derma roller needle length, such as 0.25mm?

derma roller

Choosing the appropriate derma roller needle length depends primarily on your skin concerns, experience level with microneedling, and sensitivity. Here are a few key guidelines we follow:

For first-time users or those with sensitive skin, a 0.25mm to 0.5mm needle length is recommended. This provides gentle exfoliation without going deep into fragile skin. Start here to assess your body’s response.

If you have used derma rollers before and want moderate skin rejuvenation, opt for 0.5mm to 1mm length. This penetrates deeper to better stimulate collagen and treat issues like scars or wrinkles.

A study by Singh and Yadav suggests that for treating acne and other scars, a needle length of 1.5–2 mm is typically used, while for treating aging skin and wrinkles, a needle length of 0.5 mm or 1.0 mm is recommended.

For advanced anti-aging effects and tissue regeneration, choose 1mm to 1.5mm needles. This delivers deeper micro punctures best suited for those experienced with microneedling. Use caution to avoid adverse effects.

Always disinfect the skin and follow a proper microneedling technique. Adjust your approach based on how your skin looks and feels after each session. Allow adequate healing time between treatments.

What is the difference between stainless steel and titanium derma rollers?

The main differences between stainless steel and titanium derma rollers are cost, durability, and potential for irritation.

Stainless steel is more affordable than titanium and provides adequate strength and hypoallergenic properties for safe microneedling. However, stainless steel is more prone to dulling over time.

Titanium has a higher upfront cost but is more durable, maintaining its sharpness 5-6 times longer than stainless steel. This generally leads to better long-term results. Titanium causes less reactivity for those sensitive to metals.

Is the 540-needle design on a stainless steel derma roller effective for skincare?

Yes, the 540-needle design on a stainless steel derma roller is highly effective for enhancing the skin’s appearance and rejuvenation. This density of needles helps cover a greater surface area while remaining comfortable.

  • A large study looked at the effects of microneedling with derma rollers on collagen and elastin production. The study included 480 participants who used derma rollers over a period of time. At the end of the study, the results showed a 60 to 80% increase in collagen and elastin fibers within the participants’ skin.
  • A systematic review on microneedling explains how the minimally invasive treatment uses devices with up to 540 tiny needles. These needles puncture into the outer and inner layers of skin, forming microscopic channels.

The small size of the microneedles allows them to penetrate down to the dermis layer beneath the epidermis. This controlled minor skin injury then leads to the release of growth factors. These include transforming growth factor alpha and beta, fibroblast growth factor, and platelet-derived growth factor.

In our experience supplying microneedling devices, 540 needles deliver optimal stimulation of new collagen with a balance of coverage and gentleness. Rollers with too few needles must be rolled more times, while excessive needles may heighten inflammation.

The 540 needles are dispersed in a uniform pattern to ensure even induction of the skin’s self-repair. This translates to consistent results across the treatment area. With multi-directional rolling, the width and spacing of the 540 needles provide a thorough microneedling effect.

How does microneedling with a 0.25mm derma roller improve skin texture and appearance?

Microneedling with a 0.25mm derma roller improves skin texture and appearance in a few key ways:

  • Exfoliates and removes dead skin cell buildup clogging pores for a refreshed, radiant complexion.
  • Creates micro-punctures in the skin that stimulate collagen production and healthy tissue regeneration.
  • Minimally invasive wounds allow enhanced absorption of serums, creams, etc.
  • New collagen plumps lines and smooths uneven scars or textures. 

In one study, patients collectively reported a 51%–60% improvement in scar appearance and a 40%–50% improvement in skin texture after microneedling.

  • Gently stimulates fibroblasts for thicker, firmer skin that retains elasticity.
  • Improved blood flow to the area delivers nutrients for anti-aging benefits.

What are the benefits of using a microneedle surgical stainless steel 540 needles derma roller with 0.25mm needles?

Based on our experience and expertise, here are some of the benefits of using a 0.25mm microneedle roller:

  • Enhanced absorption of topical skincare products with gentle microchannels. 

A study claimed a 100,000 times increase in the flux of drugs following derma roller treatment, indicating an increase in the absorption of applied compounds.

  • Improved skin texture and brightness from exfoliation of dead skin cells
  • Collagen induction and elastin stimulation for firmer, thicker, more youthful skin
  • Softening the appearance of fine lines, wrinkles, and enlarged pores
  • Reduction of acne scars and smoothing of uneven texture or stretch marks
  • Increased blood flow to nourish skin cells and promote healing
  • Minimal pain and downtime suitable for sensitive skin types
  • Effective and safe for continued use from the comfort of home
  • Affordable investment compared to professional microneedling
  • Easy to incorporate into your regular beauty regimen for gradual improvements

Are there any specific skin concerns that a 0.25mm derma roller is best suited for?

a matured woman touches her smooth skin

At 0.25mm, derma rollers are ideal for addressing early signs of aging, enlarged pores, acne scarring, and uneven texture. This length works well for delicate areas like around the eyes. Those with thin or reactive skin also do better with shorter needle sizes.

Some specific skin concerns best addressed with a 0.25mm derma roller include:

  • Minimizing the appearance of pores

A study published in the Biomedical Microdevices journal found that derma rollers can increase the permeation of applied formulations to the site of action within the skin, which can help minimize the appearance of pores.

  • Softening crow’s feet, smile lines, and forehead creases

A study published in the Aesthetic Surgery Journal found that microneedling is a viable, minimally invasive option for treating wrinkles of the neck, which can also be applied to facial wrinkles.

  • Lessening acne scars, especially rolling or boxcar scars
  • Fading stretch marks from pregnancy, weight fluctuations, or growth spurts


A review of applications of microneedling in dermatology found that microneedling has shown promising results in the treatment of atrophic scars, which can include stretch marks.

  • Smoothing uneven texture and tone for a glowing complexion

For issues like deep wrinkles, volume loss, or advanced aging, we typically recommend graduating to a longer needle length after assessing skin tolerance. Always monitor for side effects and adjust accordingly.

What skincare products should be used in combination with a 0.25mm derma roller for best results?

To maximize your results, we advise applying targeted skincare serums or treatments immediately after using your derma roller when absorption is highest. Here are some products we recommend:

  • Hyaluronic acid serums to hydrate and plump skin while repairing the micro-punctures. Look for low molecular weights.
  • Antioxidants like vitamin C to protect, brighten, and reduce inflammation induced by microneedling.
  • Peptides or growth factors to further stimulate collagen and skin cell renewal.
  • Retinoids to boost cell turnover and regeneration, improving tone and texture over time. Use lower strengths.

A study on using microneedling with retinoic acid for melasma, published on PubMed, looked at the combined treatment’s efficacy. The researchers found that after 60 days, both microneedling and retinoic acid demonstrated effectiveness in improving facial melasma. 

However, the study also highlighted that combining microneedling with retinoic acid could potentially decrease antioxidants that protect the skin against oxidative stress damage.

  • Moisturizers rich in nourishing oils or ceramides to reinforce the skin barrier as it heals.
  • Always avoid harsh actives like retinols, AHAs, or benzoyl peroxide for 24 hours after microneedling. Nourishing, soothing ingredients work best. Introduce actives gradually as your skin adjusts.

How often should I use a 540-needle surgical stainless steel derma roller with 0.25mm needles for skincare?

When first starting out with a 0.25mm derma roller, we suggest using it once every 2-4 weeks to allow your skin to get comfortable with the micro-injuries. As your skin builds tolerance, you can increase usage to once every 7-14 days.

For maintenance, after you’ve achieved desired results, 1-2 times per month is often sufficient. Always listen to your skin – if it feels sensitized or irritated, allow more healing time between sessions. Some people use their derma roller as frequently as once a week with no adverse effects.

What is the recommended technique for using a 540-needle surgical stainless steel derma roller with 0.25mm needles?

Proper 0.25mm derma roller technique involves cleansing skin, disinfecting the device before and after use, rolling lightly in multiple directions without overdoing any area, avoiding sensitive spots, applying serums immediately after, thoroughly cleaning the derma roller, and avoiding actives post-treatment.

  1. Always start with a freshly cleansed face – use a gentle cleanser suited for your skin type.
  2. Sterilize your derma roller before each use with isopropyl alcohol – allow it to fully dry.
  3. If desired, apply a numbing cream 30 minutes prior to reduce discomfort.
  4. Start at the center of the face and roll outwards towards the hairline using light pressure. Apply moderate pressure as your skin adjusts.
  5. Roll in multiple directions – up and down, side to side, and diagonally. Perform each pass only 6-8 times.

Research by Singh and Yadav examined the microneedling technique using derma rollers. Their study found that rolling a standard derma roller over the skin 15 times in one area creates approximately 250 micro channels per square centimeter into the upper dermis layer. The depth reached into the dermis depends on the amount of pressure applied while rolling.

  1. Avoid going over the same area repeatedly during one session to minimize damage.

A study published on StatPearls notes that microneedling should be avoided on active acne, inflammatory lesions, or other localized infections in the skin. Performing microneedling over these affected areas is not recommended.

  1. Do not roll over active breakouts, moles, or extremely sensitive areas. Use around eyes cautiously.
  2. Apply serums, mists, and moisturizers immediately after microneedling when absorption is maximized.
  3. Clean the derma roller thoroughly after each use and store in a sterilized protective case.
  4. Continue your regular skincare regimen, avoiding actives like retinol for 24 hours post-treatment.

Are there any specific precautions or aftercare steps when using a 0.25mm derma roller?

While 0.25mm derma rollers are generally safe for at-home use, following precautions and proper aftercare remains important:

  • Have a clean, sterile derma roller and disinfected skin to avoid infection risk.

A study published in PubMed Central highlights the importance of utilizing a sterile derma roller to prevent infection. The needles on derma rollers are pre-sterilized using gamma irradiation, and these devices are designed for single use only. This emphasizes the need to use a sterile derma roller and avoid reusing it to minimize infection risks.

  • Use a light touch initially if new to microneedling – you can always increase pressure.
  • Apply SPF 30+ daily after use, as increased sun sensitivity is common.

Healthline recommends avoiding sun exposure after microneedling and using sunscreen to protect the skin.

  • Avoid picking or scrubbing the skin post-treatment to minimize scarring.
  • Use only gentle, non-irritating cleansers and moisturizers in your aftercare regimen.
  • If using actives like retinol or vitamin C, introduce them gradually to avoid irritation.

According to Healthline, it is recommended to avoid using retinol and vitamin C serums for at least 48 hours after microneedling. After this initial period, these skincare products can be slowly introduced back into the routine. Allowing the skin some time to heal before applying these potent actives helps minimize irritation risks.

  • Hydrate skin well and avoid heat, sweating, swimming until skin fully recovers.
  • Watch for signs of infection or excessive redness – seek medical care if concerns arise.
  • Allow downtime for cellular regeneration before your next microneedling session.

With the proper precautions, 0.25mm derma rollers can be used safely and effectively at home. Adjust pressure and frequency based on your skin’s response.

Can you use a 0.25mm derma roller at home, or should it be done by a professional?

The short 0.25mm needle length is well suited for at-home use with proper technique and aftercare. The gentle micro punctures typically do not require professional expertise. However, we do recommend consulting a dermatologist if:

  • You have very sensitive skin, a history of keloids, open wounds, or eczema.
  • Your skin condition doesn’t improve within 2-3 months of using the derma roller.
  • You experience excessive pain, irritation, scarring, or hyperpigmentation.
  • You require a deeper treatment for advanced signs of aging or sagging skin.

Licensed estheticians can safely use longer needle lengths of up to 3mm for enhanced remodeling. But for basic exfoliation and collagen stimulation, a 0.25mm derma roller works well alone at home when following our guidance. Monitor your skin’s response closely.

Are there any potential side effects or risks associated with using a 0.25mm derma roller?

When used correctly, side effects from a 0.25mm derma roller are minimal. Potential risks include:

  • Temporary redness, irritation, dryness as the skin recovers.

A systematic review studying the potential side effects of microneedling treatments found that skin redness was a common occurrence. This was true regardless of the type of microneedling device used. The review was conducted by researchers who examined current literature on microneedling and complications that can happen with this procedure. 

Their analysis of multiple studies showed erythema, or redness, frequently results from the micro-injuries created during microneedling. This review demonstrates skin redness is a common side effect to be aware of with this procedure.

  • Mild swelling, bruising, or minor pinpoint bleeding if pressed too hard.

The same systematic review also found that edema (swelling) was more likely to occur with the roller than with other microneedling devices. The study also mentioned that bleeding was a common side effect of using a roller microneedling device.

  • Infection from an unsterilized derma roller – use alcohol to disinfect.
  • Scarring if used excessively without allowing proper healing between sessions.
  • Hyperpigmentation or dark spots if skin is exposed to sun too soon after microneedling.


The above systematic review found that postinflammatory hyperpigmentation was more likely to occur with the roller and radiofrequency microneedling devices than with the stamp.

  • Allergic reaction in those sensitive to metals like nickel (rare with surgical steel).

The same review also mentioned that roller microneedling devices may contain nickel and various other metals, thus contact allergies may occur.

With appropriate precautions, technique, and aftercare, these risks are low. Adjust usage based on your skin’s feedback and tolerance. Seek medical help for signs of infection or adverse reactions.

Where can I purchase a microneedle surgical stainless steel 540 needles derma roller with 0.25mm needles?

At Face Med Store, we offer high-quality surgical steel derma rollers with 540 needles in various sizes, including the 0.25mm length for home use. We also carry titanium rollers and dermapens for enhanced longevity. 

You can also find microneedling devices through other trusted online retailers, local med spas, dermatology clinics, or aesthetician offices. Be sure to purchase from authorized sellers to get an authentic device that is FDA-approved. Beware of non-sterile or low-quality rollers that could damage skin.

Save on Microneedling Essentials – Shop Our Catalog Now

a portrait of a smiling, beautiful woman

Microneedling with a 0.25mm, 540-needle stainless steel derma roller offers a safe, effective way to boost collagen, refine texture, minimize scars, and reveal brighter, more youthful-looking skin. By following our guidance on proper technique, aftercare, and application, you can incorporate this treatment into your skincare routine for noticeable improvements with minimal risk.

At Face Med Store, we offer a range of medical-grade microneedling products to meet your skincare goals. We hope this guide gives you the knowledge needed to revitalize your complexion from the comfort of your home. Let us know if you have any other questions!

All content in this blog is for informational purposes only. It is not medical or legal advice. Please consult with lawyer or a medical professional.